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Increased retention of functional fusions to toxic genes in new two-hybrid libraries of the E. coli strain MG1655 and B. subtilis strain 168 genomes, prepared without passaging through E. coli
BACKGROUND: Cloning of genes in expression libraries, such as the yeast two-hybrid system (Y2H), is based on the assumption that the loss of target genes is minimal, or at worst, managable. However, the expression of genes or gene fragments that are capable of interacting with E. coli or yeast gene...
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| Main Authors: | , , , , , |
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| Formato: | Artigo |
| Idioma: | en |
| Publicado em: |
BioMed Central
2003
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| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC212392/ https://ncbi.nlm.nih.gov/pubmed/12964949 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2164-4-36 |
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