Purification and Some Properties of Carbon Monoxide Dehydrogenase from Pseudomonas carboxydohydrogena

A soluble yellow CO dehydrogenase from CO-autotrophically grown cells of Pseudomonas carboxydohydrogena was purified 35-fold in seven steps to better than 95% homogeneity with a yield of 30%. The final specific activity was 180 μmol of acceptor reduced per min per mg of protein as determined by an a...

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Main Authors: Kim, Y. M., Hegeman, G. D., Jacques, N A, Wittenberger, C L, Liao, H H, Thorner, J, Jackowski, S, Rock, C O, Stirling, L A, Ahmad, P M, Ahmad, F, Hall, C V, Yanofsky, C, Waxman, D J, Lindgren, D M, Strominger, J L, Stark, A A, Yankofsky, S A, Suzuki, T, Iino, T, Gupta, Manju, Carr, Noel G., Yamamoto, T, Yokota, T, Kaji, A, Niwa, O, Sakaguchi, K, Gunge, N, Kim, Y M, Hegeman, G D, Hedstrom, R C, Schockley, R K, Eagon, R G, Sutton, Ann, Newman, Thomas, Francis, Marilyn, Freundlich, Martin, Kane, J F, Wakim, J, Fischer, R S, Filer, D, Furano, A V, Lipsky, R H, Rosenthal, R, Zahler, S A, Zurkowski, W, Salyers, Abigail A., O'Brien, Mildred, Kotarski, Susan F., Morona, R, Reeves, P, Crow, V L, Thomas, T D, Roy, N K, Ghosh, R K, Das, J, Ritzenthaler, P, Mata-Gilsinger, M, Trueba, F J, Neijssel, O M, Woldringh, C L, Roberton, A M, Glucina, P G, Rode, Hergo, Giffhorn, Friedrich, Mock, Michèle, Pugsley, Anthony P., Diaz, R, Staudenbauer, W L, Simon, J P, Wargnies, B, Stalon, V, Kerber, N L, Cardenas, J, Henderson, Gary B., Potuznik, Suzana, Lindberg, R A, Rhodes, W G, Eirich, L D, Drucker, H, Sugio, T, Kudo, S, Tano, T, Imai, K, Oudega, B, Stegehuis, F, van Tiel-Menkveld, G J, de Graaf, F K, Gowrishankar, J, Pittard, J, Linz, J E, Orlowski, M, Inamine, G S, Niederman, R A, Schweizer, Herbert, Argast, Manfred, Boos, Winfried, Schweizer, H, Grussenmeyer, T, Boos, W, Ougham, H J, Trudgill, P W, Hellingwerf, K J, Friedberg, I, Lolkema, J S, Michels, P A, Konings, W N, Imhoff, J F, Kushner, D J, Kushwaha, S C, Kates, M, Primerano, Donald A., Burns, R. O., Polacheck, I, Hearing, V J, Kwon-Chung, K J, Gray, G L, Berka, R M, Vasil, M L, Moore, C W
Formato: Artigo
Idioma:English
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Publicado em: 1981
Assuntos:
Enzymology
Research Article
Genetics and Molecular Biology
Errata
Physiology and Metabolism
Plasmids and Transposons
Membranes
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC216291/
https://ncbi.nlm.nih.gov/pubmed/6895515
https://ncbi.nlm.nih.gov/pmc/articles/PMC216292/
https://ncbi.nlm.nih.gov/pubmed/7309680
https://ncbi.nlm.nih.gov/pmc/articles/PMC216293/
https://ncbi.nlm.nih.gov/pubmed/6171560
https://ncbi.nlm.nih.gov/pmc/articles/PMC216294/
https://ncbi.nlm.nih.gov/pubmed/6796563
https://ncbi.nlm.nih.gov/pmc/articles/PMC216295/
https://ncbi.nlm.nih.gov/pubmed/6796564
https://ncbi.nlm.nih.gov/pmc/articles/PMC216296/
https://ncbi.nlm.nih.gov/pubmed/6171561
https://ncbi.nlm.nih.gov/pmc/articles/PMC216297/
https://ncbi.nlm.nih.gov/pubmed/6796565
https://ncbi.nlm.nih.gov/pmc/articles/PMC216298/
https://ncbi.nlm.nih.gov/pubmed/7309681
https://ncbi.nlm.nih.gov/pmc/articles/PMC216299/
https://ncbi.nlm.nih.gov/pubmed/7309682
https://ncbi.nlm.nih.gov/pmc/articles/PMC216300/
https://ncbi.nlm.nih.gov/pubmed/7309683
https://ncbi.nlm.nih.gov/pmc/articles/PMC216301/
https://ncbi.nlm.nih.gov/pubmed/6118357
https://ncbi.nlm.nih.gov/pmc/articles/PMC216302/
https://ncbi.nlm.nih.gov/pubmed/7031037
https://ncbi.nlm.nih.gov/pmc/articles/PMC216303/
https://ncbi.nlm.nih.gov/pubmed/7309684
https://ncbi.nlm.nih.gov/pmc/articles/PMC216304/
https://ncbi.nlm.nih.gov/pubmed/6273386
https://ncbi.nlm.nih.gov/pmc/articles/PMC216305/
https://ncbi.nlm.nih.gov/pubmed/6796566
https://ncbi.nlm.nih.gov/pmc/articles/PMC216306/
https://ncbi.nlm.nih.gov/pubmed/7031038
https://ncbi.nlm.nih.gov/pmc/articles/PMC216307/
https://ncbi.nlm.nih.gov/pubmed/6118356
https://ncbi.nlm.nih.gov/pmc/articles/PMC216308/
https://ncbi.nlm.nih.gov/pubmed/6796560
https://ncbi.nlm.nih.gov/pmc/articles/PMC216309/
https://ncbi.nlm.nih.gov/pubmed/6796561
https://ncbi.nlm.nih.gov/pmc/articles/PMC216310/
https://ncbi.nlm.nih.gov/pmc/articles/PMC216315/
https://ncbi.nlm.nih.gov/pubmed/7076625
https://ncbi.nlm.nih.gov/pmc/articles/PMC216316/
https://ncbi.nlm.nih.gov/pubmed/6804433
https://ncbi.nlm.nih.gov/pmc/articles/PMC216317/
https://ncbi.nlm.nih.gov/pubmed/6281230
https://ncbi.nlm.nih.gov/pmc/articles/PMC216318/
https://ncbi.nlm.nih.gov/pubmed/6281231
https://ncbi.nlm.nih.gov/pmc/articles/PMC216319/
https://ncbi.nlm.nih.gov/pubmed/6804434
https://ncbi.nlm.nih.gov/pmc/articles/PMC216320/
https://ncbi.nlm.nih.gov/pubmed/6281232
https://ncbi.nlm.nih.gov/pmc/articles/PMC216321/
https://ncbi.nlm.nih.gov/pubmed/6804435
https://ncbi.nlm.nih.gov/pmc/articles/PMC216322/
https://ncbi.nlm.nih.gov/pubmed/6122675
https://ncbi.nlm.nih.gov/pmc/articles/PMC216323/
https://ncbi.nlm.nih.gov/pubmed/6978882
https://ncbi.nlm.nih.gov/pmc/articles/PMC216324/
https://ncbi.nlm.nih.gov/pubmed/6281233
https://ncbi.nlm.nih.gov/pmc/articles/PMC216325/
https://ncbi.nlm.nih.gov/pubmed/6281234
https://ncbi.nlm.nih.gov/pmc/articles/PMC216326/
https://ncbi.nlm.nih.gov/pubmed/6281235
https://ncbi.nlm.nih.gov/pmc/articles/PMC216327/
https://ncbi.nlm.nih.gov/pubmed/6978883
https://ncbi.nlm.nih.gov/pmc/articles/PMC216328/
https://ncbi.nlm.nih.gov/pubmed/6804436
https://ncbi.nlm.nih.gov/pmc/articles/PMC216329/
https://ncbi.nlm.nih.gov/pubmed/6210687
https://ncbi.nlm.nih.gov/pmc/articles/PMC216330/
https://ncbi.nlm.nih.gov/pubmed/6804437
https://ncbi.nlm.nih.gov/pmc/articles/PMC216331/
https://ncbi.nlm.nih.gov/pubmed/6281236
https://ncbi.nlm.nih.gov/pmc/articles/PMC216332/
https://ncbi.nlm.nih.gov/pubmed/6281237
https://ncbi.nlm.nih.gov/pmc/articles/PMC216333/
https://ncbi.nlm.nih.gov/pubmed/7042684
https://ncbi.nlm.nih.gov/pmc/articles/PMC216334/
https://ncbi.nlm.nih.gov/pubmed/7076616
https://ncbi.nlm.nih.gov/pmc/articles/PMC216335/
https://ncbi.nlm.nih.gov/pubmed/6804438
https://ncbi.nlm.nih.gov/pmc/articles/PMC216336/
https://ncbi.nlm.nih.gov/pubmed/7042685
https://ncbi.nlm.nih.gov/pmc/articles/PMC216337/
https://ncbi.nlm.nih.gov/pubmed/6281238
https://ncbi.nlm.nih.gov/pmc/articles/PMC216338/
https://ncbi.nlm.nih.gov/pubmed/7076617
https://ncbi.nlm.nih.gov/pmc/articles/PMC216339/
https://ncbi.nlm.nih.gov/pubmed/6281239
https://ncbi.nlm.nih.gov/pmc/articles/PMC216340/
https://ncbi.nlm.nih.gov/pubmed/7076618
https://ncbi.nlm.nih.gov/pmc/articles/PMC216341/
https://ncbi.nlm.nih.gov/pubmed/7042686
https://ncbi.nlm.nih.gov/pmc/articles/PMC216342/
https://ncbi.nlm.nih.gov/pubmed/6804439
https://ncbi.nlm.nih.gov/pmc/articles/PMC216343/
https://ncbi.nlm.nih.gov/pubmed/6804440
https://ncbi.nlm.nih.gov/pmc/articles/PMC216344/
https://ncbi.nlm.nih.gov/pubmed/7042687
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https://ncbi.nlm.nih.gov/pmc/articles/PMC216291/
https://ncbi.nlm.nih.gov/pubmed/6895515
https://ncbi.nlm.nih.gov/pmc/articles/PMC216292/
https://ncbi.nlm.nih.gov/pubmed/7309680
https://ncbi.nlm.nih.gov/pmc/articles/PMC216293/
https://ncbi.nlm.nih.gov/pubmed/6171560
https://ncbi.nlm.nih.gov/pmc/articles/PMC216294/
https://ncbi.nlm.nih.gov/pubmed/6796563
https://ncbi.nlm.nih.gov/pmc/articles/PMC216295/
https://ncbi.nlm.nih.gov/pubmed/6796564
https://ncbi.nlm.nih.gov/pmc/articles/PMC216296/
https://ncbi.nlm.nih.gov/pubmed/6171561
https://ncbi.nlm.nih.gov/pmc/articles/PMC216297/
https://ncbi.nlm.nih.gov/pubmed/6796565
https://ncbi.nlm.nih.gov/pmc/articles/PMC216298/
https://ncbi.nlm.nih.gov/pubmed/7309681
https://ncbi.nlm.nih.gov/pmc/articles/PMC216299/
https://ncbi.nlm.nih.gov/pubmed/7309682
https://ncbi.nlm.nih.gov/pmc/articles/PMC216300/
https://ncbi.nlm.nih.gov/pubmed/7309683
https://ncbi.nlm.nih.gov/pmc/articles/PMC216301/
https://ncbi.nlm.nih.gov/pubmed/6118357
https://ncbi.nlm.nih.gov/pmc/articles/PMC216302/
https://ncbi.nlm.nih.gov/pubmed/7031037
https://ncbi.nlm.nih.gov/pmc/articles/PMC216303/
https://ncbi.nlm.nih.gov/pubmed/7309684
https://ncbi.nlm.nih.gov/pmc/articles/PMC216304/
https://ncbi.nlm.nih.gov/pubmed/6273386
https://ncbi.nlm.nih.gov/pmc/articles/PMC216305/
https://ncbi.nlm.nih.gov/pubmed/6796566
https://ncbi.nlm.nih.gov/pmc/articles/PMC216306/
https://ncbi.nlm.nih.gov/pubmed/7031038
https://ncbi.nlm.nih.gov/pmc/articles/PMC216307/
https://ncbi.nlm.nih.gov/pubmed/6118356
https://ncbi.nlm.nih.gov/pmc/articles/PMC216308/
https://ncbi.nlm.nih.gov/pubmed/6796560
https://ncbi.nlm.nih.gov/pmc/articles/PMC216309/
https://ncbi.nlm.nih.gov/pubmed/6796561
https://ncbi.nlm.nih.gov/pmc/articles/PMC216310/
https://ncbi.nlm.nih.gov/pmc/articles/PMC216315/
https://ncbi.nlm.nih.gov/pubmed/7076625
https://ncbi.nlm.nih.gov/pmc/articles/PMC216316/
https://ncbi.nlm.nih.gov/pubmed/6804433
https://ncbi.nlm.nih.gov/pmc/articles/PMC216317/
https://ncbi.nlm.nih.gov/pubmed/6281230
https://ncbi.nlm.nih.gov/pmc/articles/PMC216318/
https://ncbi.nlm.nih.gov/pubmed/6281231
https://ncbi.nlm.nih.gov/pmc/articles/PMC216319/
https://ncbi.nlm.nih.gov/pubmed/6804434
https://ncbi.nlm.nih.gov/pmc/articles/PMC216320/
https://ncbi.nlm.nih.gov/pubmed/6281232
https://ncbi.nlm.nih.gov/pmc/articles/PMC216321/
https://ncbi.nlm.nih.gov/pubmed/6804435
https://ncbi.nlm.nih.gov/pmc/articles/PMC216322/
https://ncbi.nlm.nih.gov/pubmed/6122675
https://ncbi.nlm.nih.gov/pmc/articles/PMC216323/
https://ncbi.nlm.nih.gov/pubmed/6978882
https://ncbi.nlm.nih.gov/pmc/articles/PMC216324/
https://ncbi.nlm.nih.gov/pubmed/6281233
https://ncbi.nlm.nih.gov/pmc/articles/PMC216325/
https://ncbi.nlm.nih.gov/pubmed/6281234
https://ncbi.nlm.nih.gov/pmc/articles/PMC216326/
https://ncbi.nlm.nih.gov/pubmed/6281235
https://ncbi.nlm.nih.gov/pmc/articles/PMC216327/
https://ncbi.nlm.nih.gov/pubmed/6978883
https://ncbi.nlm.nih.gov/pmc/articles/PMC216328/
https://ncbi.nlm.nih.gov/pubmed/6804436
https://ncbi.nlm.nih.gov/pmc/articles/PMC216329/
https://ncbi.nlm.nih.gov/pubmed/6210687
https://ncbi.nlm.nih.gov/pmc/articles/PMC216330/
https://ncbi.nlm.nih.gov/pubmed/6804437
https://ncbi.nlm.nih.gov/pmc/articles/PMC216331/
https://ncbi.nlm.nih.gov/pubmed/6281236
https://ncbi.nlm.nih.gov/pmc/articles/PMC216332/
https://ncbi.nlm.nih.gov/pubmed/6281237
https://ncbi.nlm.nih.gov/pmc/articles/PMC216333/
https://ncbi.nlm.nih.gov/pubmed/7042684
https://ncbi.nlm.nih.gov/pmc/articles/PMC216334/
https://ncbi.nlm.nih.gov/pubmed/7076616
https://ncbi.nlm.nih.gov/pmc/articles/PMC216335/
https://ncbi.nlm.nih.gov/pubmed/6804438
https://ncbi.nlm.nih.gov/pmc/articles/PMC216336/
https://ncbi.nlm.nih.gov/pubmed/7042685
https://ncbi.nlm.nih.gov/pmc/articles/PMC216337/
https://ncbi.nlm.nih.gov/pubmed/6281238
https://ncbi.nlm.nih.gov/pmc/articles/PMC216338/
https://ncbi.nlm.nih.gov/pubmed/7076617
https://ncbi.nlm.nih.gov/pmc/articles/PMC216339/
https://ncbi.nlm.nih.gov/pubmed/6281239
https://ncbi.nlm.nih.gov/pmc/articles/PMC216340/
https://ncbi.nlm.nih.gov/pubmed/7076618
https://ncbi.nlm.nih.gov/pmc/articles/PMC216341/
https://ncbi.nlm.nih.gov/pubmed/7042686
https://ncbi.nlm.nih.gov/pmc/articles/PMC216342/
https://ncbi.nlm.nih.gov/pubmed/6804439
https://ncbi.nlm.nih.gov/pmc/articles/PMC216343/
https://ncbi.nlm.nih.gov/pubmed/6804440
https://ncbi.nlm.nih.gov/pmc/articles/PMC216344/
https://ncbi.nlm.nih.gov/pubmed/7042687

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