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Design, total chemical synthesis, and binding properties of a [Leu-91-N(1)-methyl-7-azaTrp]Ras-binding domain of c-Raf-1
The Ras-binding domain (RBD) of c-Raf-1 has been synthesized chemically, taking advantage of the chemical ligation of two peptide fragments of the protein. This procedure allowed incorporation of an unnatural amino acid (N(1)-methyl-7-azatryptophan) at position 91 of RBD, producing a protein with fl...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | en |
| Publicado em: |
The National Academy of Sciences
1999
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC22153/ https://ncbi.nlm.nih.gov/pubmed/10393913 |
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