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Structural investigations of the active-site mutant Asn156Ala of outer membrane phospholipase A: Function of the Asn–His interaction in the catalytic triad

Outer membrane phospholipase A (OMPLA) from Escherichia coli is an integral-membrane enzyme with a unique His–Ser–Asn catalytic triad. In serine proteases and serine esterases usually an Asp occurs in the catalytic triad; its role has been the subject of much debate. Here the role of the uncharged a...

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Detalhes bibliográficos
Main Authors: Snijder, H.J., Van Eerde, J.H., Kingma, R.L., Kalk, K.H., Dekker, N., Egmond, M.R., Dijkstra, B.W.
Formato: Artigo
Idioma:English
Publicado em: Cold Spring Harbor Laboratory Press 2001
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2374211/
https://ncbi.nlm.nih.gov/pubmed/11567087
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