Validation of a HLA-A2 tetramer flow cytometric method, IFNgamma real time RT-PCR, and IFNgamma ELISPOT for detection of immunologic response to gp100 and MelanA/MART-1 in melanoma patients

BACKGROUND: HLA-A2 tetramer flow cytometry, IFNγ real time RT-PCR and IFNγ ELISPOT assays are commonly used as surrogate immunological endpoints for cancer immunotherapy. While these are often used as research assays to assess patient's immunologic response, assay validation is necessary to ens...

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Detalhes bibliográficos
Main Authors: Xu, Yuanxin, Theobald, Valerie, Sung, Crystal, DePalma, Kathleen, Atwater, Laura, Seiger, Keirsten, Perricone, Michael A, Richards, Susan M
Formato: Artigo
Idioma:English
Publicado em: BioMed Central 2008
Assuntos:
Research
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2605437/
https://ncbi.nlm.nih.gov/pubmed/18945350
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1479-5876-6-61
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Internet

https://ncbi.nlm.nih.gov/pmc/articles/PMC2605437/
https://ncbi.nlm.nih.gov/pubmed/18945350
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1479-5876-6-61

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