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Cost-effective HRMA pre-sequence typing of clone libraries; application to phage display selection

BACKGROUND: Methodologies like phage display selection, in vitro mutagenesis and the determination of allelic expression differences include steps where large numbers of clones need to be compared and characterised. In the current study we show that high-resolution melt curve analysis (HRMA) is a si...

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Detalhes bibliográficos
Main Authors: Pepers, Barry A, Schut, Menno H, Vossen, Rolf HAM, van Ommen, Gert-Jan B, den Dunnen, Johan T, van Roon-Mom, Willeke MC
Formato: Artigo
Idioma:English
Publicado em: BioMed Central 2009
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2694173/
https://ncbi.nlm.nih.gov/pubmed/19463169
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1472-6750-9-50
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