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Circular Polymerase Extension Cloning of Complex Gene Libraries and Pathways
High-throughput genomics and the emerging field of synthetic biology demand ever more convenient, economical, and efficient technologies to assemble and clone genes, gene libraries and synthetic pathways. Here, we describe the development of a novel and extremely simple cloning method, circular poly...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | English |
| Publicado em: |
Public Library of Science
2009
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2713398/ https://ncbi.nlm.nih.gov/pubmed/19649325 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0006441 |
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