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Modulation of the stability of a gene-regulatory protein dimer by DNA and cAMP.

We describe an experimental approach to the measurement of protein subunit exchange in which biotinylated subunits mediate attachment of 35S-labeled subunits to a streptavidin column as a result of the exchange process. Application of the method to Escherichia coli catabolite activator protein (CAP)...

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Detalhes bibliográficos
Main Authors: Brown, A M, Crothers, D M
Formato: Artigo
Idioma:en
Publicado em: 1989
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC298067/
https://ncbi.nlm.nih.gov/pubmed/2552443
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