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Codon cassette mutagenesis: a general method to insert or replace individual codons by using universal mutagenic cassettes.
We describe codon cassette mutagenesis, a simple method of mutagenesis that uses universal mutagenic cassettes to deposit single codons at specific sites in double-stranded DNA. A target molecule is first constructed that contains a blunt, double-strand break at the site targeted for mutagenesis. A...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | en |
| Publicado em: |
1994
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| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC308034/ https://ncbi.nlm.nih.gov/pubmed/8202358 |
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