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Antibody Engineering Using Phage Display with a Coiled-Coil Heterodimeric Fv Antibody Fragment
A Fab-like antibody binding unit, ccFv, in which a pair of heterodimeric coiled-coil domains was fused to V(H) and V(L) for Fv stabilization, was constructed for an anti-VEGF antibody. The anti-VEGF ccFv showed the same binding affinity as scFv but significantly improved stability and phage display...
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| Formato: | Artigo |
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| Idioma: | en |
| Publicado em: |
Public Library of Science
2011
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| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3084267/ https://ncbi.nlm.nih.gov/pubmed/21552519 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0019023 |
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