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HisB from Mycobacterium tuberculosis: cloning, overexpression in Mycobacterium smegmatis, purification, crystallization and preliminary X-ray crystallographic analysis

HisB, encoded by open reading frame Rv1601, possesses enzymatic activity as an imidazoleglycerol-phosphate dehydratase in the histidine-biosynthetic pathway of Mycobacterium tuberculosis. A recombinant form of HisB was crystallized in three crystal forms: crystals grown using 20% PEG 1500 as a preci...

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Bibliografiske detaljer
Format: Artigo
Sprog:en
Udgivet: International Union of Crystallography 2011
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Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3212474/
https://ncbi.nlm.nih.gov/pubmed/22102255
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309111037201
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