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Identification of gene products programmed by restriction endonuclease DNA fragments using an E. coli in vitro system.

DNA restriction enzyme fragments have been used to programme the synthesis of polypeptides in an in vitro system without apparent loss in fidelity compared with supercoiled templates. The system is extremely sensitive, less than 1 microgram of DNA can be used to direct the synthesis of 35S-labelled...

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Detalhes bibliográficos
Main Authors: Pratt, J M, Boulnois, G J, Darby, V, Orr, E, Wahle, E, Holland, I B
Formato: Artigo
Idioma:en
Publicado em: 1981
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC327450/
https://ncbi.nlm.nih.gov/pubmed/6272207
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