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Alterations of RNase H sensitivity of the 3' splice site region during the in vitro splicing reaction.
We have developed a splicing assay system with an immobilized pre-mRNA to study the mechanism of the splicing reaction after spliceosome assembly. Using this system, we have found that the second step of the splicing reaction could be dissected into two stages. After the 5' splice site reaction...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | en |
| Publicado em: |
1991
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| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC328488/ https://ncbi.nlm.nih.gov/pubmed/1650457 |
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