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Chemical and enzymatic biotin-labeling of oligodeoxyribonucleotides.
Biotin has been converted to 2-(biotinylamido)ethanol and condensed to phosphorylated oligonucleotides in a solid phase synthesis. The 5'-biotinylated oligonucleotides were enzymatically coupled to other DNA fragments by T4 DNA ligase or T4 RNA ligase. The hybridization properties of such bioti...
Sábháilte in:
| Príomhchruthaitheoirí: | , , , |
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| Formáid: | Alt |
| Teanga: | en |
| Foilsithe / Cruthaithe: |
1985
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| Rochtain ar líne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC340973/ https://ncbi.nlm.nih.gov/pubmed/2582352 |
| Clibeanna: |
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