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Modification of the melting properties of duplex DNA by attachment of a GC-rich DNA sequence as determined by denaturing gradient gel electrophoresis.

The melting behavior of a DNA fragment carrying the mouse beta maj-globin promoter was investigated as a means of establishing procedures for separating DNA fragments differing by any single base substitution using the denaturing gradient gel electrophoresis procedure of Fischer and Lerman (1,2). We...

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Detalhes bibliográficos
Main Authors: Myers, R M, Fischer, S G, Maniatis, T, Lerman, L S
Formato: Artigo
Idioma:en
Publicado em: 1985
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC341224/
https://ncbi.nlm.nih.gov/pubmed/2987873
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