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Simplified methods for large scale enzymatic synthesis of oligoribonucleotides.
We report simplified methods for large scale enzymatic synthesis of oligoribonucleotides using polynucleotide phosphorylase. The main features of the method are use of RPC-5 chromatography, including chromatography at two pH values to deal with the problem of primer phosphorolysis, rapid dialysis fo...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | en |
| Publicado em: |
1978
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| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC342164/ https://ncbi.nlm.nih.gov/pubmed/673855 |
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