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White and green screening with circular polymerase extension cloning for easy and reliable cloning
Cloning is an essential prerequisite to test protein design and engineering ideas. However, it is often time consuming, unreliable, and therefore frustrating. Here, we present a streamlined cloning strategy that incorporates a powerful white and green screening protocol to identify colonies with ins...
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| Formato: | Artigo |
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| Idioma: | en |
| Publicado em: |
Blackwell Publishing Ltd
2013
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3690724/ https://ncbi.nlm.nih.gov/pubmed/23592493 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pro.2268 |
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