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Ligand occupancy mimicked by single residue substitutions in a receptor: transmembrane signaling induced by mutation.
We used mixed, mutagenic oligonucleotides to create single amino acid substitutions in the bacterial chemoreceptor Trg. Mutagenesis was directed at a 20-residue segment of the periplasmic domain implicated in ligand recognition. Transmembrane signaling by the mutant receptors was assayed in vivo by...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | en |
| Publicado em: |
1992
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC49820/ https://ncbi.nlm.nih.gov/pubmed/1518809 |
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