A novel strategy for the identification of protein–DNA contacts by photocrosslinking and mass spectrometry

Photochemical crosslinking is a method for studying the molecular details of protein–nucleic acid interactions. In this study, we describe a novel strategy to localize crosslinked amino acid residues that combines laser-induced photocrosslinking, proteolytic digestion, Fe(3+)-IMAC (immobilized metal...

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Bibliographische Detailangaben
Hauptverfasser: Geyer, Hildegard, Geyer, Rudolf, Pingoud, Vera
Format: Artikel
Sprache:English
Veröffentlicht: Oxford University Press 2004
Schlagworte:
NAR Methods Online
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC519130/
https://ncbi.nlm.nih.gov/pubmed/15383647
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gnh131
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https://ncbi.nlm.nih.gov/pmc/articles/PMC519130/
https://ncbi.nlm.nih.gov/pubmed/15383647
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gnh131

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