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Selective ‘stencil’-aided pre-PCR cleavage of wild-type sequences as a novel approach to detection of mutant K-RAS
The enriched PCR widely used for detection of mutant K-RAS in either tumor tissues or circulating DNA was modified so that abundant wild-type K-RAS alleles are cleaved prior to PCR. We took advantage of an AluI recognition site located immediately upstream of the K-RAS codon 12. The site was reconst...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | en |
| Publicado em: |
Oxford University Press
2001
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC55902/ https://ncbi.nlm.nih.gov/pubmed/11522846 |
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