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Cloning, Expression, and Purification of the K5 Capsular Polysaccharide Lyase (KflA) from Coliphage K5A: Evidence for Two Distinct K5 Lyase Enzymes

The Escherichia coli K5 capsular polysaccharide [-4)-βGlcA-(1,4)-αGlcNAc-(1-] is a receptor for the capsule-specific bacteriophage K5A. Associated with the structure of bacteriophage K5A is a polysaccharide lyase which degrades the K5 capsule to expose the underlying bacterial cell surface. The bact...

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Detalhes bibliográficos
Main Authors:	Clarke, Bradley R., Esumeh, Fred, Roberts, Ian S., Hahn, Ji-Sook, Oh, So-Young, Roe, Jung-Hye, Wang, Hongmei, Roberts, Adam P., Lyras, Dena, Rood, Julian I., Wilks, Mark, Mullany, Peter, Martin, Vincent J. J., Mohn, William W., Bennik, Marjon H. J., Pomposiello, Pablo J., Thorne, Derek F., Demple, Bruce, Dubrac, Sarah, Touati, Danièle, Auerbach, Ilene D., Sorensen, Cody, Hansma, Helen G., Holden, Patricia A., Muscholl-Silberhorn, Albrecht B., Kamath, Shilpa, Chen, M. L., Chakrabarty, A. M., Zahrt, Thomas C., Deretic, Vojo, Ochoa de Alda, Jesús A. G., Ajlani, Ghada, Houmard, Jean, Jander, Georg, Rahme, Laurence G., Ausubel, Frederick M., Mongkolsuk, Skorn, Whangsuk, Wirongrong, Fuangthong, Mayuree, Loprasert, Suvit, Hannibal, Laure, Lorquin, Jean, D'Ortoli, Nicolas Angles, Garcia, Nelly, Chaintreuil, Clemence, Masson-Boivin, Catherine, Dreyfus, Bernard, Giraud, Eric, Maier, R. J., Moshiri, F., Kwon, Ohsuk, Georgellis, Dimitris, Lin, E. C. C., Winstedt, Lena, Frankenberg, Lena, Hederstedt, Lars, von Wachenfeldt, Claes, Wernegreen, Jennifer J., Ochman, Howard, Jones, Isaac B., Moran, Nancy A., Wehrl, Wolfgang, Niederweis, Michael, Schumann, Wolfgang, Aguirre, Andrés, Lejona, Sergio, Véscovi, Eleonora García, Soncini, Fernando C., Varon, Mazal, Choder, Mordechai, Guo, Ming, Sun, Zhonghe, Zhang, Ying, Zhu, Jun, Oger, Philippe M., Schrammeijer, Barbara, Hooykaas, Paul J. J., Farrand, Stephen K., Winans, Stephen C., Skovran, Elizabeth, Downs, Diana M., Martín, Mauricio, Magni, Christian, López, Paloma, de Mendoza, Diego, Aarons, Simon, Abbas, Abdelhamid, Adams, Claire, Fenton, Anne, O'Gara, Fergal, Hu, Biao, Zhu, Jiabi, Shen, San Chiun, Yu, Guan-qiao, Youngren, Brenda, Radnedge, Lyndsay, Hu, Ping, Garcia, Emilio, Austin, Stuart, Xue, Hong, Guo, Rong, Wen, Yunfei, Liu, Danxu, Huang, Li, Hager, Paul W., Calfee, M. Worth, Phibbs, Paul V., Hiratsuka, Koji, Reha-Krantz, Linda J., Smeets, Leonard C., Bijlsma, Jetta J. E., Boomkens, Sacha Y., Vandenbroucke-Grauls, Christina M. J. E., Kusters, Johannes G., Fournier, Bénédicte, Hooper, David C., Hu, Zonglin, Lutkenhaus, Joe, Praszkier, J., Murthy, S., Pittard, A. J., Konan, Kouacou Vincent, Yanofsky, Charles, Schupp, James M., Klevytska, Alexandra M., Zinser, Guenevier, Price, Lance B., Keim, Paul, Momma, Keiko, Okamoto, Masako, Mishima, Yumiko, Mori, Shigetarou, Hashimoto, Wataru, Murata, Kousaku, Vierling, Silke, Weber, Tilmann, Wohlleben, Wolfgang, Muth, Günther, Hamrick, Terri S., Harris, Sandra L., Spears, Patricia A., Havell, Edward A., Horton, John R., Russell, Perry W., Orndorff, Paul E., Fekete, Richard A., Frost, Laura S., White, E. Lucile, Ross, Larry J., Reynolds, Robert C., Seitz, Lainne E., Moore, Georgia D., Borhani, David W., Cheng, Qi, Paszkiet, Brian J., Shoemaker, Nadja B., Gardner, Jeffrey F., Salyers, Abigail A., Bonifield, Heather R., Yamaguchi, Shigeru, Hughes, Kelly T., Voulhoux, Romé, Taupiac, Marie-Pierre, Czjzek, Mirjam, Beaumelle, Bruno, Filloux, Alain, Vilchèze, Catherine, Morbidoni, Hector R., Weisbrod, Torin R., Iwamoto, Hiroyuki, Kuo, Mack, Sacchettini, James C., Jacobs, William R., Feilmeier, Bradley J., Iseminger, Ginger, Schroeder, Diane, Webber, Hannali, Phillips, Gregory J., Guina, Tina, Yi, Eugene C., Wang, Houle, Hackett, Murray, Miller, Samuel I., Li, Rongfeng, Khaleeli, Nusrat, Townsend, Craig A., Sato, Hisaaki, Watanabe, Takao, Higuchi, Kohichi, Teruya, Kuniaki, Ohtake, Ayumi, Murata, Yasuko, Saito, Hiroshi, Aizawa, Chikara, Danbara, Hirofumi, Maehara, Nobutoshi
Formato:	Artigo
Idioma:	English English English English English English English English English English English English English English English English English English English English English English English English English English English English English English English English English English English English English English English English English English English English English English English English English English
Publicado em:	American Society for Microbiology 2000
Assuntos:	Bacteriophages Genetics and Molecular Biology Plasmids and Transposons Models and Cell Growth Physiology and Metabolism Structure and Function Population Genetics and Evolution Eukaryotic Cells Enzymes and Proteins Minireviews Plant Microbiology Cell Surfaces
Acesso em linha:	https://ncbi.nlm.nih.gov/pmc/articles/PMC94548/ https://ncbi.nlm.nih.gov/pubmed/10850992 https://ncbi.nlm.nih.gov/pmc/articles/PMC94549/ https://ncbi.nlm.nih.gov/pubmed/10850993 https://ncbi.nlm.nih.gov/pmc/articles/PMC94550/ https://ncbi.nlm.nih.gov/pubmed/10850994 https://ncbi.nlm.nih.gov/pmc/articles/PMC94551/ https://ncbi.nlm.nih.gov/pubmed/10850995 https://ncbi.nlm.nih.gov/pmc/articles/PMC94552/ https://ncbi.nlm.nih.gov/pubmed/10850996 https://ncbi.nlm.nih.gov/pmc/articles/PMC94553/ https://ncbi.nlm.nih.gov/pubmed/10850997 https://ncbi.nlm.nih.gov/pmc/articles/PMC94554/ https://ncbi.nlm.nih.gov/pubmed/10850998 https://ncbi.nlm.nih.gov/pmc/articles/PMC94555/ https://ncbi.nlm.nih.gov/pubmed/10850999 https://ncbi.nlm.nih.gov/pmc/articles/PMC94556/ https://ncbi.nlm.nih.gov/pubmed/10851000 https://ncbi.nlm.nih.gov/pmc/articles/PMC94557/ https://ncbi.nlm.nih.gov/pubmed/10851001 https://ncbi.nlm.nih.gov/pmc/articles/PMC94558/ https://ncbi.nlm.nih.gov/pubmed/10851002 https://ncbi.nlm.nih.gov/pmc/articles/PMC94559/ https://ncbi.nlm.nih.gov/pubmed/10851003 https://ncbi.nlm.nih.gov/pmc/articles/PMC94560/ https://ncbi.nlm.nih.gov/pubmed/10851004 https://ncbi.nlm.nih.gov/pmc/articles/PMC94561/ https://ncbi.nlm.nih.gov/pubmed/10851005 https://ncbi.nlm.nih.gov/pmc/articles/PMC94562/ https://ncbi.nlm.nih.gov/pubmed/10851006 https://ncbi.nlm.nih.gov/pmc/articles/PMC94563/ https://ncbi.nlm.nih.gov/pubmed/10851007 https://ncbi.nlm.nih.gov/pmc/articles/PMC94564/ https://ncbi.nlm.nih.gov/pubmed/10851008 https://ncbi.nlm.nih.gov/pmc/articles/PMC94565/ https://ncbi.nlm.nih.gov/pubmed/10851009 https://ncbi.nlm.nih.gov/pmc/articles/PMC94566/ https://ncbi.nlm.nih.gov/pubmed/10851010 https://ncbi.nlm.nih.gov/pmc/articles/PMC94567/ https://ncbi.nlm.nih.gov/pubmed/10851011 https://ncbi.nlm.nih.gov/pmc/articles/PMC94568/ https://ncbi.nlm.nih.gov/pubmed/10851012 https://ncbi.nlm.nih.gov/pmc/articles/PMC94569/ https://ncbi.nlm.nih.gov/pubmed/10851013 https://ncbi.nlm.nih.gov/pmc/articles/PMC94570/ https://ncbi.nlm.nih.gov/pubmed/10869063 https://ncbi.nlm.nih.gov/pmc/articles/PMC94571/ https://ncbi.nlm.nih.gov/pubmed/10869064 https://ncbi.nlm.nih.gov/pmc/articles/PMC94572/ https://ncbi.nlm.nih.gov/pubmed/10869065 https://ncbi.nlm.nih.gov/pmc/articles/PMC94573/ https://ncbi.nlm.nih.gov/pubmed/10869066 https://ncbi.nlm.nih.gov/pmc/articles/PMC94574/ https://ncbi.nlm.nih.gov/pubmed/10869067 https://ncbi.nlm.nih.gov/pmc/articles/PMC94575/ https://ncbi.nlm.nih.gov/pubmed/10869068 https://ncbi.nlm.nih.gov/pmc/articles/PMC94576/ https://ncbi.nlm.nih.gov/pubmed/10869069 https://ncbi.nlm.nih.gov/pmc/articles/PMC94577/ https://ncbi.nlm.nih.gov/pubmed/10869070 https://ncbi.nlm.nih.gov/pmc/articles/PMC94578/ https://ncbi.nlm.nih.gov/pubmed/10869071 https://ncbi.nlm.nih.gov/pmc/articles/PMC94579/ https://ncbi.nlm.nih.gov/pubmed/10869072 https://ncbi.nlm.nih.gov/pmc/articles/PMC94580/ https://ncbi.nlm.nih.gov/pubmed/10869073 https://ncbi.nlm.nih.gov/pmc/articles/PMC94581/ https://ncbi.nlm.nih.gov/pubmed/10869074 https://ncbi.nlm.nih.gov/pmc/articles/PMC94582/ https://ncbi.nlm.nih.gov/pubmed/10869075 https://ncbi.nlm.nih.gov/pmc/articles/PMC94583/ https://ncbi.nlm.nih.gov/pubmed/10869076 https://ncbi.nlm.nih.gov/pmc/articles/PMC94584/ https://ncbi.nlm.nih.gov/pubmed/10869077 https://ncbi.nlm.nih.gov/pmc/articles/PMC94585/ https://ncbi.nlm.nih.gov/pubmed/10869078 https://ncbi.nlm.nih.gov/pmc/articles/PMC94586/ https://ncbi.nlm.nih.gov/pubmed/10869079 https://ncbi.nlm.nih.gov/pmc/articles/PMC94587/ https://ncbi.nlm.nih.gov/pubmed/10869080 https://ncbi.nlm.nih.gov/pmc/articles/PMC94588/ https://ncbi.nlm.nih.gov/pubmed/10869081 https://ncbi.nlm.nih.gov/pmc/articles/PMC94589/ https://ncbi.nlm.nih.gov/pubmed/10869082 https://ncbi.nlm.nih.gov/pmc/articles/PMC94590/ https://ncbi.nlm.nih.gov/pubmed/10869083 https://ncbi.nlm.nih.gov/pmc/articles/PMC94591/ https://ncbi.nlm.nih.gov/pubmed/10869084 https://ncbi.nlm.nih.gov/pmc/articles/PMC94592/ https://ncbi.nlm.nih.gov/pubmed/10869085 https://ncbi.nlm.nih.gov/pmc/articles/PMC94593/ https://ncbi.nlm.nih.gov/pubmed/10869086 https://ncbi.nlm.nih.gov/pmc/articles/PMC94594/ https://ncbi.nlm.nih.gov/pubmed/10869087 https://ncbi.nlm.nih.gov/pmc/articles/PMC94595/ https://ncbi.nlm.nih.gov/pubmed/10869088 https://ncbi.nlm.nih.gov/pmc/articles/PMC94596/ https://ncbi.nlm.nih.gov/pubmed/10869089 https://ncbi.nlm.nih.gov/pmc/articles/PMC94597/ https://ncbi.nlm.nih.gov/pubmed/10869090
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Cloning, Expression, and Purification of the K5 Capsular Polysaccharide Lyase (KflA) from Coliphage K5A: Evidence for Two Distinct K5 Lyase Enzymes

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