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A Truncated Soluble Bacillus Signal Peptidase Produced in Escherichia coli Is Subject to Self-Cleavage at Its Active Site

Soluble forms of Bacillus signal peptidases which lack their unique amino-terminal membrane anchor are prone to degradation, which precludes their high-level production in the cytoplasm of Escherichia coli. Here, we show that the degradation of soluble forms of the Bacillus signal peptidase SipS is...

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Hlavní autoři:	van Roosmalen, M. L., Jongbloed, J. D. H., Kuipers, A., Venema, G., Bron, S., van Dijl, J. M., Crick, Dean C., Schulbach, Mark C., Zink, Erin E., Macchia, Marco, Barontini, Silvia, Besra, Gurdyal S., Brennan, Patrick J., Gon, Stéphanie, Patte, Jean-Claude, Méjean, Vincent, Iobbi-Nivol, Chantal, Poggio, Sebastian, Aguilar, Carlos, Osorio, Aurora, González-Pedrajo, Bertha, Dreyfus, Georges, Camarena, Laura, Yang, Zhaomin, Ma, Xiaoyuan, Tong, Leming, Kaplan, Heidi B., Shimkets, Lawrence J., Shi, Wenyuan, Leboeuf, Céline, Leblanc, Laurence, Auffray, Yanick, Hartke, Axel, Cho, Eun Hee, Alcaraz, Renato, Gumport, Richard I., Gardner, Jeffrey F., Wang, Henian, Gunsalus, Robert P., São-José, Carlos, Parreira, Ricardo, Vieira, Graça, Santos, Mário A., Temmerman, Wim, Vereecke, Danny, Dreesen, Rozemarijn, Van Montagu, Marc, Holsters, Marcelle, Goethals, Koen, Cunningham, Francis X., Lafond, Toulouse P., Gantt, Elisabeth, Breinig, Sabine, Schiltz, Emile, Fuchs, Georg, Munson, George P., Lam, Deborah L., Outten, F. Wayne, O'Halloran, Thomas V., Boni, Irina V., Artamonova, Valentina S., Dreyfus, Marc, Franco, Alejandro, Soto, Teresa, Vicente-Soler, Jero, Guillen, Pedro Valero, Cansado, Jose, Gacto, Mariano, Mansilla, Maria Cecilia, Albanesi, Daniela, de Mendoza, Diego, Chakrabarti, Swarup K., Misra, Tapan K., Onogi, Toshinari, Yamazoe, Mitsuyoshi, Ichinose, Chiyome, Niki, Hironori, Hiraga, Sota, Antoine, Rudy, Alonso, Sylvie, Raze, Dominique, Coutte, Loïc, Lesjean, Sarah, Willery, Eve, Locht, Camille, Jacob-Dubuisson, Françoise, Tamaru, Yutaka, Karita, Shuichi, Ibrahim, Atef, Chan, Helen, Doi, Roy H., Sanchez, Rony, Roovers, Martine, Glansdorff, Nicolas, Handrick, René, Reinhardt, Simone, Jendrossek, Dieter, Zähner, Dorothea, Hakenbeck, Regine, Matic, Ivan, Taddei, François, Radman, Miroslav, Pink, David, Moeller, Jeremy, Quinn, Bonnie, Jericho, Manfred, Beveridge, Terry, Borezee, Elise, Msadek, Tarek, Durant, Lionel, Berche, Patrick, Silhavy, Thomas J., Belitsky, Boris R., Wray, Lewis V., Fisher, Susan H., Bohannon, Dian E., Sonenshein, Abraham L., Bereswill, Stefan, Greiner, Stefan, van Vliet, Arnoud H. M., Waidner, Barbara, Fassbinder, Frank, Kusters, Johannes G., Kist, Manfred, Cadieux, Nathalie, Bradbeer, Clive, Kadner, Robert J., Yu, Sung-Liang, Ko, Kai-Liang, Chen, Chang-Shi, Chang, Yu-Chung, Syu, Wan-Jr, Gilmour, Raymond, Messner, Paul, Guffanti, Arthur A., Kent, Rebecca, Scheberl, Andrea, Kendrick, Nancy, Krulwich, Terry Ann, van den Bogaard, Patrick T. C., Kleerebezem, Michiel, Kuipers, Oscar P., de Vos, Willem M., Köhler, Thilo, Curty, Lasta Kocjancic, Barja, Francisco, van Delden, Christian, Pechère, Jean-Claude, Vignais, Paulette M., Dimon, Bernard, Zorin, Nikolay A., Tomiyama, Masamitsu, Colbeau, Annette, St. Geme, Joseph W., Cutter, David, Rosche, Thomas M., Siddique, Azeem, Larsen, Michelle H., Figurski, David H., Artsimovitch, Irina, Svetlov, Vladimir, Anthony, Larry, Burgess, Richard R., Landick, Robert, Hopfner, Karl-Peter, Karcher, Annette, Shin, David, Fairley, Cecilia, Tainer, John A., Carney, James P., Alexandre, Gladys, Greer, Suzanne E., Zhulin, Igor B., Holland-Staley, Carol A., Lee, KangSeok, Clark, David P., Cunningham, Philip R., Boyd, David A., Cvitkovitch, Dennis G., Bleiweis, Arnold S., Kiriukhin, Michael Y., Debabov, Dmitri V., Neuhaus, Francis C., Hamilton, Ian R., Kropinski, Andrew M., Gründling, Angelika, Smith, David L., Bläsi, Udo, Young, Ry, Freestone, Primrose P. E., Lyte, Mark, Neal, Christopher P., Maggs, Anthony F., Haigh, Richard D., Williams, Peter H., Warner, Jessica B., Krom, Bastiaan P., Magni, Christian, Konings, Wil N., Lolkema, Juke S., Walker, James R., Hervas, Christine, Ross, Julie D., Blinkova, Alexandra, Walbridge, Michael J., Pumarega, Emilynn J., Park, Mi-Oak, Neely, Harold R., Krásný, Libor, Vacík, Tomáš, Fučík, Vladimír, Jonák, Jiří, Contzen, Matthias, Stolz, Andreas
Médium:	Článek
Jazyk:	English English English English English English English English English English English English English English English English English English English English English English English English English English English English English English English English English English English English English English English English English English English English English English English English English English
Vydáno:	American Society for Microbiology 2000
Témata:	Enzymes and Proteins Physiology and Metabolism Genetics and Molecular Biology Cell Surfaces Bacteriophages Plant Microbiology Eukaryotic Cells Guest Commentary Structure and Function Plasmids and Transposons
On-line přístup:	https://ncbi.nlm.nih.gov/pmc/articles/PMC94698/ https://ncbi.nlm.nih.gov/pubmed/11004175 https://ncbi.nlm.nih.gov/pmc/articles/PMC94699/ https://ncbi.nlm.nih.gov/pubmed/11004176 https://ncbi.nlm.nih.gov/pmc/articles/PMC94700/ https://ncbi.nlm.nih.gov/pubmed/11004177 https://ncbi.nlm.nih.gov/pmc/articles/PMC94701/ https://ncbi.nlm.nih.gov/pubmed/11004178 https://ncbi.nlm.nih.gov/pmc/articles/PMC94702/ https://ncbi.nlm.nih.gov/pubmed/11004179 https://ncbi.nlm.nih.gov/pmc/articles/PMC94703/ https://ncbi.nlm.nih.gov/pubmed/11004180 https://ncbi.nlm.nih.gov/pmc/articles/PMC94704/ https://ncbi.nlm.nih.gov/pubmed/11004181 https://ncbi.nlm.nih.gov/pmc/articles/PMC94705/ https://ncbi.nlm.nih.gov/pubmed/11004182 https://ncbi.nlm.nih.gov/pmc/articles/PMC94706/ https://ncbi.nlm.nih.gov/pubmed/11004183 https://ncbi.nlm.nih.gov/pmc/articles/PMC94707/ https://ncbi.nlm.nih.gov/pubmed/11004184 https://ncbi.nlm.nih.gov/pmc/articles/PMC94708/ https://ncbi.nlm.nih.gov/pubmed/11004185 https://ncbi.nlm.nih.gov/pmc/articles/PMC94709/ https://ncbi.nlm.nih.gov/pubmed/11004186 https://ncbi.nlm.nih.gov/pmc/articles/PMC94710/ https://ncbi.nlm.nih.gov/pubmed/11004187 https://ncbi.nlm.nih.gov/pmc/articles/PMC94711/ https://ncbi.nlm.nih.gov/pubmed/11004188 https://ncbi.nlm.nih.gov/pmc/articles/PMC94712/ https://ncbi.nlm.nih.gov/pubmed/11004189 https://ncbi.nlm.nih.gov/pmc/articles/PMC94713/ https://ncbi.nlm.nih.gov/pubmed/11004190 https://ncbi.nlm.nih.gov/pmc/articles/PMC94714/ https://ncbi.nlm.nih.gov/pubmed/11004191 https://ncbi.nlm.nih.gov/pmc/articles/PMC94715/ https://ncbi.nlm.nih.gov/pubmed/11004192 https://ncbi.nlm.nih.gov/pmc/articles/PMC94716/ https://ncbi.nlm.nih.gov/pubmed/11004193 https://ncbi.nlm.nih.gov/pmc/articles/PMC94717/ https://ncbi.nlm.nih.gov/pubmed/11004194 https://ncbi.nlm.nih.gov/pmc/articles/PMC94718/ https://ncbi.nlm.nih.gov/pubmed/11004195 https://ncbi.nlm.nih.gov/pmc/articles/PMC94719/ https://ncbi.nlm.nih.gov/pubmed/11004196 https://ncbi.nlm.nih.gov/pmc/articles/PMC94720/ https://ncbi.nlm.nih.gov/pubmed/11004197 https://ncbi.nlm.nih.gov/pmc/articles/PMC94721/ https://ncbi.nlm.nih.gov/pubmed/11004198 https://ncbi.nlm.nih.gov/pmc/articles/PMC94722/ https://ncbi.nlm.nih.gov/pubmed/11004199 https://ncbi.nlm.nih.gov/pmc/articles/PMC94723/ https://ncbi.nlm.nih.gov/pubmed/11004200 https://ncbi.nlm.nih.gov/pmc/articles/PMC94724/ https://ncbi.nlm.nih.gov/pubmed/11029410 https://ncbi.nlm.nih.gov/pmc/articles/PMC94725/ https://ncbi.nlm.nih.gov/pubmed/11029411 https://ncbi.nlm.nih.gov/pmc/articles/PMC94726/ https://ncbi.nlm.nih.gov/pubmed/11029412 https://ncbi.nlm.nih.gov/pmc/articles/PMC94727/ https://ncbi.nlm.nih.gov/pubmed/11029413 https://ncbi.nlm.nih.gov/pmc/articles/PMC94728/ https://ncbi.nlm.nih.gov/pubmed/11029414 https://ncbi.nlm.nih.gov/pmc/articles/PMC94729/ https://ncbi.nlm.nih.gov/pubmed/11029415 https://ncbi.nlm.nih.gov/pmc/articles/PMC94730/ https://ncbi.nlm.nih.gov/pubmed/11029416 https://ncbi.nlm.nih.gov/pmc/articles/PMC94731/ https://ncbi.nlm.nih.gov/pubmed/11029417 https://ncbi.nlm.nih.gov/pmc/articles/PMC94732/ https://ncbi.nlm.nih.gov/pubmed/11029418 https://ncbi.nlm.nih.gov/pmc/articles/PMC94733/ https://ncbi.nlm.nih.gov/pubmed/11029419 https://ncbi.nlm.nih.gov/pmc/articles/PMC94734/ https://ncbi.nlm.nih.gov/pubmed/11029420 https://ncbi.nlm.nih.gov/pmc/articles/PMC94735/ https://ncbi.nlm.nih.gov/pubmed/11029421 https://ncbi.nlm.nih.gov/pmc/articles/PMC94736/ https://ncbi.nlm.nih.gov/pubmed/11029422 https://ncbi.nlm.nih.gov/pmc/articles/PMC94737/ https://ncbi.nlm.nih.gov/pubmed/11029423 https://ncbi.nlm.nih.gov/pmc/articles/PMC94738/ https://ncbi.nlm.nih.gov/pubmed/11029424 https://ncbi.nlm.nih.gov/pmc/articles/PMC94739/ https://ncbi.nlm.nih.gov/pubmed/11029425 https://ncbi.nlm.nih.gov/pmc/articles/PMC94740/ https://ncbi.nlm.nih.gov/pubmed/11029426 https://ncbi.nlm.nih.gov/pmc/articles/PMC94741/ https://ncbi.nlm.nih.gov/pubmed/11029427 https://ncbi.nlm.nih.gov/pmc/articles/PMC94742/ https://ncbi.nlm.nih.gov/pubmed/11029428 https://ncbi.nlm.nih.gov/pmc/articles/PMC94743/ https://ncbi.nlm.nih.gov/pubmed/11029429 https://ncbi.nlm.nih.gov/pmc/articles/PMC94744/ https://ncbi.nlm.nih.gov/pubmed/11029430 https://ncbi.nlm.nih.gov/pmc/articles/PMC94745/ https://ncbi.nlm.nih.gov/pubmed/11029431 https://ncbi.nlm.nih.gov/pmc/articles/PMC94746/ https://ncbi.nlm.nih.gov/pubmed/11029432 https://ncbi.nlm.nih.gov/pmc/articles/PMC94747/ https://ncbi.nlm.nih.gov/pubmed/11029433
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A Truncated Soluble Bacillus Signal Peptidase Produced in Escherichia coli Is Subject to Self-Cleavage at Its Active Site

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